Figure 2

Dicer's helicase domain is required for co-immunoprecipitating Tat. A) Co-immunoprecipations were performed after transfection of Dicer mutants deleted from the N-terminus progressively to encompass the DEAD domain (ΔDEAD), the helicase domain (ΔHelicase), the Domain of Unknown Function 283 (ΔDUF), and the PAZ domain (ΔPAZ) as schematically illustrated in panel B. Cell lysates (lanes 7 to 12) and immunoprecipitations using anti-flag beads were characterized by immunoblotting using anti-flag (upper panel), anti-Tat (middle panel) or anti-Ago2 (bottom panel). B) Schematic illustration of the Dicer mutants and summary of the co-immunoprecipitation between Dicer and Tat and Dicer and TRBP.