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Figure 3 | Retrovirology

Figure 3

From: The strength of the HIV-1 3' splice sites affects Rev function

Figure 3

Weakening of the 5'ss D4 does not compensate for the strength of 3'ss A7. HeLa-T4+ cells were transiently transfected with the subgenomic HIV-1 constructs (SV E/X tat - rev -) combining an efficient (A7+) or inefficient (A7) 3'ss with a 5'ss with high (D4) or lower (-1G3U, cf. Fig. 2A) complementarity to U1 snRNA. The p(A)+ RNA was analyzed by Northern blotting (cf. Fig. 2). (A) Northern blot with indication of the ratio of spliced (s) and unspliced (us) RNA in presence of Rev ([s/us], mean ± standard error) from three independent experiments. (B) Mean values of the relative amounts of spliced (s, black) and unspliced (us, grey) transcripts from three independent experiments, normalized to transcription efficiency (hGH). The spliced (s) and unspliceds (us) RNA populations were quantified from different exposure times of the blots to adjust for the different levels of signal intensities. The maximum values of both RNA populations were defined as 100%.

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