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Table 1 Primers used to amplify regions of NF90ctv by PCR for cloning into the pCI-neo vector.

From: Nuclear Factor 90, a cellular dsRNA binding protein inhibits the HIV Rev-export function

 

Amplified region

Construct

5'-primer: 5'GGGGGGGAATTCGCTAGCCGCCACCAATGCAGAAGACGGAGCACATGAC3'

3'-primer: 5'GGGGGGCCCGGGGCGGCCGACTAGATGGTCAGGGACAATGGACGCTCTTT3'

NES

pCI-neo/NES NF90

5'-primer: 5'GGGGGGGAATTCGCTAGCCGCCACCAATGAATGCCCTGATGCGGTTGAA3'

3'-primer: 5'GGGGGGCCCGGGGCGGCCGACTAAGCACCCGTCGGCAAGCCCATGTCCTG3'

DRBD 1

pCI-neo/DRBD 1 NF90

5'-primer: 5'GGGGGGGAATTCGCTAGCCGCCACCAATGAAGAACCCAGTCATGGAGCTG3'

3-primer: 5'GGGGGGCCCGGGGCGGCCGACTAAGGGAAAAGTTTTTCTAGGGCAGCAAG3'

DRBD 2

pCI-neo/DRBD 2 NF90

5'-primer: 5'GGGGGGGTCGACCGCCACCAATGAAGCTTTTCCCTGACACCCCTCTCGCC3'

3'-primer: 5'GGGGGGCCCGGGCGGCCGCCTATGTAGCCTCCATGGTTGGCGCCACCAAA3'

RG-

pCI-neo/RG- NF90