Figure 2
From: Nuclear Factor 90, a cellular dsRNA binding protein inhibits the HIV Rev-export function
Effect of NF90ctv protein domains on the level of expression of CAT. HeLa cells were cotransfected with CAT-RRE, and with different constructs that code for Rev protein and the NF90ctv domains. After cell lysis, 14C-labelled chloramphenicol and acetyl CoA were added to the extracts and the CAT activity determined. The construct included in each assay is indicated above the lanes. Vertical arrows highlight the constructs leading to the strongest decrease in acetylation of chloramphenicol. Similar results were obtained in three independent experiments. When the cells were transfected only with the empty expression vector for NF90 (pCI-neo), acetylation of chloramphenicol was not observed (not shown).