Table 1 Determination of sites of phosphorylation in Tat
From: Phosphorylation of HIV-1 Tat by CDK2 in HIV-1 transcription
Peak | Peptides matching to Tat | MW | -18Da | Matching peptides |
|---|---|---|---|---|
I | (T)RKGLGISYG(R) | 950.606 | 932.606 | 932.606 931.553 |
| Â | (L)GISYGRKKRR/(Q) | 1220.7 | 1202.7 | 1202.7 1202.698 |
| Â | (S)YGRKKRRQRR/(R) | 1403.779 | 1385.779 | 1385.779 1385.777 |
II | (L)EPWKHPGSQPRTACNNCYCK/(K) | 2319.272 | 2301.272 | 2301.272 2301.274 |
| Â | (P)WKHPGSQPRTAC(N) | 1367.563 | 1349.563 | 1349.563 1349.544 1349.563 1349.544 |
| Â | (P)WKHPGSQPRTACNN(C) | 1595.811 | 1577.811 | 1577.811, 1576.779 |
| Â | (P)NLEPWKHPG(S) | 1077.606 | 1059.606 | 1059.606 |