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Figure 6 | Retrovirology

Figure 6

From: HIV-1 infection and CD4 T cell depletion in the humanized Rag2-/-γc-/-(RAG-hu) mouse model

Figure 6

CD4 T cell depletion in peripheral blood of HIV-1 infected RAG-hu mice. Peripheral blood was collected at different weeks post-infection and cells were stained with CD3 and CD4 antibodies and FACS analyzed. To determine the levels of CD4 T cells in the whole T cell population (stained with the pan T cell marker CD3), CD4:CD3 ratios were determined as described in methods. To obtain a baseline CD4:CD3 level for each individual mouse prior to HIV-1 infection, mice were bled a minimum of two times before infection. CD4 T cell levels are depicted as a percent of individual mouse baseline levels recorded at 1 week pre-infection. Shown are mean uninfected mouse levels (A, n = 4), infection with HIV-1 NL4-3 + NLENG1-IRES (B), infection with HIV-1 NLENG1-IRES alone (C), and infection with HIV-1 BaL (D). Also shown (E) are representative FACS plots from mouse #16 from various time points post-infection indicating the CD3CD4+/+ and CD3CD4+/- populations used to calculate the values shown in A-D.

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