Figure 5From: HIV-1 infection and CD4 T cell depletion in the humanized Rag2-/-γc-/-(RAG-hu) mouse modelDetection of HIV-1 in infected RAG-hu mouse tissues by in situ hybridization. Thymus and spleen were collected at 12 weeks post-infection and sections were made from the frozen tissues. In situ hybridization was performed using digoxigenin-labeled antisense probes to detect HIV-1 RNA as described in methods. Dark staining cells indicate the presence of HIV-1.Back to article page