Figure 3
From: HIV-1 infection and CD4 T cell depletion in the humanized Rag2-/-γc-/-(RAG-hu) mouse model
Ex vivo productive HIV-1 infection in human cells differentiated in reconstituted Rag2-/-γc-/- mice. Thymus, spleen and lymph node tissues were collected at 16 weeks post-engraftment. Single cell suspensions were made and stimulated for 3 days with PHA and IL-2, and later challenged with HIV-1 NL4-3 HSA reporter virus. To detect productive viral infection, culture supernatants were analyzed by p24 ELISA at different days post-infection.