Figure 4
Contemporaneous and non-contemporaneous plasma neutralization activity against infant viral isolates was determined in TZM-bl cells. Panel A shows the results of the test plasma against infant 2 and 6 or 8-month viral isolates from two rapid progressors (1449 and 2669). Panel B shows the results of the test plasma against infant 4 or 6, 12 and 48-month viral isolates from two slow progressors (1984 and 1084). The test plasma was diluted to 1:20. Virus production in the supernatants was monitored by luciferase activity at 2 days post infection. Luciferase activity in the control wells containing no plasma was defined as 100%, and the neutralization capacity of the test plasma was calculated relative to this value.