Figure 3
Dlg1 knockdown does not augment IL-2-independent cell growth induced by Tax1ΔC. (A) CTLL-2 cells were infected with a lentivirus encoding Tax1ΔC. Forty-eight hours after infection, cell lysates were prepared and the amount of Tax1ΔC in the lysates was measured by Western blot analysis using an anti-Tax1 antibody. (B) CTLL-2 cells (Dlg1-1, Dlg1-3, CAT, LUC) infected with Tax1ΔC-virus were washed twice with PBS, seeded into 96-well plates at 5 × 103 cells per well and cultured in the absence of IL-2 for four weeks. The number of wells containing outgrowing cells was counted under a light microscope. Transformation efficiency (%) was calculated as a ratio of the number of positive wells out of 96 wells. Error bars indicate standard deviations in three independent experiments.