Figure 2

Biochemical interaction of CypA and FIV CA. (A) GST-CypA or control GST fusion proteins were generated in 293T cells. After the incubation of the 293T cell lysates with glutathione beads, the beads were washed to remove unbound proteins, and then incubated with FIV virions. After removing unbound FIV virions by washing, beads were subjected to the SDS-PAGE and probed with anti-FIV CA antibodies for the detection of the interaction between CypA and FIV CA. (B) was conducted with the same experimental protocol for (A), but with different amounts of input FIV virions (serial dilution) in the presence or absence of 10 μM of CsA.