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Figure 3 | Retrovirology

Figure 3

From: 5' long terminal repeat (LTR)-selective methylation of latently infected HIV-1 provirus that is demethylated by reactivation signals

Figure 3

Identification of the flanking genomic sequence of the integrated HIV provirus in ACH-2 cells by the inverse PCR. A. A schematic chart of the inverse PCR procedure. B. Host genomic sequence flanking 5'-LTR. Underlined sequence indicates TthHB8I restriction enzyme site. Double underline indicates the sequence used for the sense primer in 5'-LTR specific methylation analysis. Bold italic indicates the L1 family sequences. Right panel shows a schematic presentation of the chromosomal location of the integration site of HIV-1 in ACH-2 cells determined by the NCBI human genomic blast program. Arrow indicates the region of integration.

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