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Figure 5 | Retrovirology

Figure 5

From: Effects of prostratin on Cyclin T1/P-TEFb function and the gene expression profile in primary resting CD4+T cells

Figure 5

Cyclin T1/P-TEFb is likely to be important for prostratin stimulation of HIV reporter virus expression. Resting CD4+ T cells were infected with wild type HIV NL4-3-Luc-Tat+ luciferase reporter virus (A) or NL4-3-Luc-Tat- (B), a mutant reporter virus with a non-functional Tat. After overnight incubation, cells were washed and cultured with DMSO or prostratin. Flavopiridol, a selective P-TEFb inhibitor, was added as indicated simultaneously with prostratin. Cells were harvest 48 hours after prostratin/flavopiridol treatment and reporter plasmid expression was examined by luciferase assays. Dashed lines indicate the background signal in the luciferase assay (~0.025) as determined from the signal in uninfected cell extract.

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