Figure 2

Effects of prostratin on the expression levels of Cyclin T1, Cyclin T2a, and CDK9. Cell extracts were prepared from resting CD4⁺ T cells from different donors cultured in DMSO or prostratin for 48 hours, and immunoblots were performed to examine the levels of Cyclin T1, CDK9, β-actin (A) and Cyclin T2a (B). The immunoblots were quantified as described in Material and Methods using β-actin for normalization; the value for fold-induction is given below the panels.