Figure 4
From: Inhibition of PP2A by LIS1 increases HIV-1 gene expression
293T cells were grown to 50% confluency and transfected with vectors expressing EGFP under the control of CMV (panel A) or PGK (panel B) promoters without or with vectors expressing LIS1 or Bγ subunit of PP2A. The EGFP expression was measured by fluorescence in the cellular lysates at 480 nm excitation and 510 nm emission as described in Methods.