Figure 3
From: Inhibition of PP2A by LIS1 increases HIV-1 gene expression
WD5 domain of LIS1 upregulates Tat mediated HIV-1 transcription. A. The WD5 domain and N-terminal domain of LIS1 were expressed in E. coli and extracted from the inclusion bodies as described in Methods. The dialyzed peptides were resolved on 12% SDS-PAGE gel and stained by Coumassie blue. B. 293 cells transfected with HIV-1 LTR-LacZ and Tat expression vectors and treated at 24 hrs posttransfection with WD5 domain or the N-terminal domain of LIS1. C, 293 cells transfected with TAR RNA-deleted HIV-1 LTR-LacZ vector and treated as in panel A. The results are presented as a fold of transactivation.