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Figure 1 | Retrovirology

Figure 1

From: Inhibition of PP2A by LIS1 increases HIV-1 gene expression

Figure 1

LIS1 induces HIV-1 transcription. Aand B, 293 cells grown in DMEM to 50% confluency were transfected with a LIS1 expression vector (panel A, lane 1), Flag-Bγ expression vector (panel B, lane 2) or pCI expression vector. Cells were lysed in SDS-loading buffer. Lysates were resolved on 12% SDS PAGE followed by immunoblotting with anti-LIS1, anti-α-tubulin or anti-Flag antibodies as indicated. C, 293 cells were grown to 50% confluency and transfected with different concentrations of vectors expressing LIS1 (lanes 3–5) or Bγ subunit of PP2A (lanes 6–8) combined with HIV-1 LTR lacZ and Tat expression vectors. The pCI-neo vector was added to keep constant the amount of CMV promoter-containing pCI vector in the transfection. Lane 1, control transfected with only HIV-1 LTR-LacZ. Lane 2, control transfected with HIV-1 LTR-LacZ and Tat expression vectors. Expression of β-galactosidase was analyzed using ONPG-based assay. The results are expressed as a fold of transactivation.

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