Figure 4From: Construction of doxycyline-dependent mini-HIV-1 variants for the development of a virotherapy against leukemiasDox regulated replication of the mini-rtTA virus rtTAΔ6A. SupT1 cells were infected with rtTAΔ6A virus (1 ng CA-p24). The culture was split and the cells were cultured with dox (upper panels) or without (lower panels). Virus replication was monitored by CA-p24 Elisa on the culture supernatant. At day 9 post infection, both cultures were washed and each culture split into one culture with dox (left panels) and one without (right panels). Filled triangles indicate cultures without dox and open triangles indicate cultures with 1000 ng/mL dox.Back to article page