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Figure 9 | Retrovirology

Figure 9

From: The conserved dileucine- and tyrosine-based motifs in MLV and MPMV envelope glycoproteins are both important to regulate a common Env intracellular trafficking

Figure 9

Interaction between GST fusions of WT or mutated MLV cytoplasmic tail and AP-1, AP-2 and AP-3 complexes. Identical quantities of GST (5 μg) (lanes 2, panels A-C), GST-MLV (lanes 3), GST-MLV-L3S (lanes 4,), GST-MLV-Y23S (lanes 5) were incubated with HeLa cell lysates (2.5 × 106 cells). The binding of AP-1, AP-2 and AP-3 complexes to GST fusion proteins was revealed by Western blotting with anti-γ adaptin mAb (panel A), anti-α adaptin mAb (panel B) and anti-δ adaptin mAb (panel C). The positions of the α-adaptin (Mr~100,000), γ-adaptin (Mr~104,000) and δ-adaptin (Mr~90,000) are indicated in the crude cell lysate from 106 cells (lanes 1).

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Retrovirology

ISSN: 1742-4690

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