Skip to main content
Figure 8 | Retrovirology

Figure 8

From: The conserved dileucine- and tyrosine-based motifs in MLV and MPMV envelope glycoproteins are both important to regulate a common Env intracellular trafficking

Figure 8

Interaction of the MLV TM-CD with μ1, μ2 and μ3 subunits in the yeast two-hybrid system. The yeast reporter strain L40 was co-transformed with plasmids encoding Gal4 AD-μ1, μ2 and μ3-adaptin (A, B and C, respectively), and plasmids encoding lexA BD fused to either the wild type (WT) or mutated (L3S, Y23S) cytoplasmic tail of MLV. Cotransformants were analyzed for histidine auxotrophy. They were patched on medium with histidine and then replica-plated on medium without histidine (- His medium). Growth in the absence of histidine indicates interaction between hybrid proteins. The positive control was the interaction between Ras and Raf proteins which bind to each other efficiently (lane 4, lower patch in panels A, B, C). Binding specificity was verified by the absence of interaction between the retroviral cytoplasmic tails (WT, L3S and Y23S) and the Gal4AD alone (none).

Back to article page

Retrovirology

ISSN: 1742-4690

Contact us