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Figure 5 | Retrovirology

Figure 5

From: The conserved dileucine- and tyrosine-based motifs in MLV and MPMV envelope glycoproteins are both important to regulate a common Env intracellular trafficking

Figure 5

Chimeras bearing the Y23S mutation are mainly relocated to a late endosomal compartment. Forty-eight hours after transfection with the Y23S mutant chimeras cDNA, HeLa cells were treated with cycloheximide for 3 hour prior to fixation and permeabilization. A. Co-staining of Y23S mutant chimeras and internalized Cy3-conjugated transferrin revealing the early/recycling endosomes.B. Before fixation, cells were allowed to take up FITC-conjugated dextran for 30 min. The cells were then extensively washed, and dextran was chased for another 30 min prior to fixation thus accumulating in late endosomal compartments. C. Co-staining of CD25 chimeras and Lamp1, a protein resident of the lysosomes.

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Retrovirology

ISSN: 1742-4690

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