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Table 1 Protease Inhibitor resistance of HIV-2 Proteases tested in yeast

From: HIV-2 Protease resistance defined in yeast cells

HIV-2ROD sequence

Phenotype (virus production)

Phenotype (transformed yeast) (% cell growth)

 

LPV

SQV

LPV

SQV

wild type

S

S

91.5+/-1.2

75.0+/-7.0

K45R

R [9]

R [9]

50.3+/-3.0

39.0+/-4.3

V20A, I54M

R [9]

R [9]

17.1+/-0.7

25.7+/-0.8

M76V

n.d.

R [10]

0.0+/- 2.4

4.0+/-0.2

  1. The susceptibility of mutated HIV-2ROD Proteases, harbouring amino acid substitutions known to confer a resistant phenotype, were assessed in yeast in the presence of LPV at 150 μM and SQV at 1mM.
  2. The resistant (R) or sensitive (S) phenotype is referred to previously published data.
  3. Results in the yeast system are presented as the percentage of cell growth; [(OD600 cells grew in Galactose with PI – OD600 cells grew in Galactose)/OD600 cells grew in Glucose] × 100.