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Figure 3 | Retrovirology

Figure 3

From: The virion-associated incoming HIV-1 RNA genome is not targeted by RNA interference

Figure 3

No sequence-specific inhibition of lentiviral transduction by RNAi. a) Schematic of lentiviral transduction. When shNef is stably produced in the target cells, the question is whether the incoming vector genome with the shNef target sequence is targeted by RNAi (dark arrow with question mark). b) SupT1 cells stably expressing shNef (+ shNef) or control SupT1 cells (- shNef) were transduced at an m.o.i. of 0.03, 0.3 or 1.0 with the control vector (JS1) or vectors containing a complete (JS1-Nef) or mutated (JS1-R2) shNef target sequence. Infected cells were analyzed by GFP-FACS. The control values (- shNef) were set at 100% for each lentiviral vector. The mean values of three experiments are shown.

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