Figure 1
From: The virion-associated incoming HIV-1 RNA genome is not targeted by RNA interference
The lentiviral vector and packaging constructs. The lentiviral vector JS1 is a third generation self-inactivating vector [39], which contains a GFP reporter gene expressed from the phosphoglycate kinase promoter (PGK) with the posttranscriptional regulatory element (pre) from hepatitis B virus. The vector genome is expressed from the Rous sarcoma promoter (RSV) and transcription starts with the R and U5 regions of the HIV-1 long terminal repeat (LTR), the packaging signal (ψ) and part of the gag open reading frame (gag). It contains the rev responsive element (RRE), central polypurine tract (cPPT) and the 3' LTR, which has a deletion in the U3 region (ΔU3). The HIV-1 sequences are tinted gray. Transcription of the vector genome and GFP reporter terminates at the HIV-1 polyA within the 3'LTR. The Nef target sequence (wild type or mutant) was cloned into the multiple cloning site (MCS). The three packaging constructs encode the trans-acting proteins required for the production of infectious virus (HIV-1 sequences in gray).