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Figure 6 | Retrovirology

Figure 6

From: HIV-1 replication in cell lines harboring INI1/hSNF5 mutations

Figure 6

Analysis of INI1 incorporation into virus particles. A. Virions produced in MON cells fail to efficiently incorporate INI1. Immunoblot analysis of highly concentrated and subtilisin treated virions produced from 293T and MON cells in the presence of increasing concentrations of INI1, using α-HA antibody (top panel) followed by α-p24 antibody (bottom panels). Bottom band in all the lanes of the top panel is due to nonspecific binding of the antibody. B. Virions produced in STA-WT1 cells efficiently incorporate INI1. Immunoblot analysis of highly concentrated and subtilisin treated virions produced from 293T and STA-WT1 cells in the presence of increasing concentrations of INI1, using α-INI1 antibody (top panel) followed by α-p24 antibody (bottom panels).

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