Figure 5

Analysis of HIV-1 particle production and infectivity of the virions from STA-WT1 cells. A. Graphic representation of virion-associated p24 antigen (pg/ml) in the culture supernatants of 293T (■) and STA-WT1 (□) cells (average of 3 independent experiments). B. Graphic representation of intracellular p24 (pg/ml) in the 293T (■), and STA-WT1 (□) cells in the presence of increasing concentrations of HA-INI1 (average of 3 independent experiments). C. Graphic representation of virion-associated p24 antigen (pg/ml) in the culture supernatants of 293T (■) and STA-WT1 (□) cells, co-transfected with the viral vectors and increasing amounts of INI1 (average of 3 independent experiments). D. The above results (B) represented as relative fold increase in particle production. E. Immunoblot analysis of relative amounts of transfected INI1 in 293T and STA-WT1 producer cells (top panel), using α-INI1 antibody. The same blot was reprobed with α-tubulin antibody as a loading control (bottom panel). F. Graphic representation of the % infectivity of particles normalized for p24, produced in 293T (■) or STA-WT1 (□) cells.