Figure 3

INI1 complements defect in the virus particle production in the MON cells in a dose-dependent manner. A. Graphic representation of intracellular p24 (pg/ml) in the 293T (■), and MON (□) cells in the presence of increasing concentrations of HA-INI1 (average of 3 independent experiments). B. Graphic representation of particle associated p24 (pg/ml) in the 293T (■), and MON (□) cells in the presence of increasing concentrations of HA-INI1 (average of 3 independent experiments). C. Immunoblot analysis of concentrated virions produced in 293T and MON cells in the presence of increasing concentrations of INI1 using α-p24 antibody. D. Immunoblot analysis of relative amounts of transfected HA-INI1 in the producer cells. The upper panel was probed with α-HA and the lower panel was probed with affinity purified α-INI1 antibodies. E. Graphic representation of fold increase in the virus particle production (■) and infectivity of virions produced in MON cells over 293T (□) and MON(....) cells. (F and G). Graphic representation of % infectivity of normalized virus particles over 293T cells (F) and MON cells (G). Virions were produced in 293T (■) or MON (□) cells in the presence of increasing concentrations of INI1 (average of 3 independent experiments).