Figure 1
From: HIV-1 replication in cell lines harboring INI1/hSNF5 mutations
Experimental Strategy used to analyze steps of HIV-1 replication in INI-deficient cells. Viral vectors pCMVΔR8.2, pMDG and pHR'-CMV-GFP were co-transfected with or without plasmid expressing HA-INI1 into 293T cells and INI-defective cells. Culture supernatants and cell lysates were examined for levels of the virus particle production and infectivity of the produced virions, incorporation of viral proteins and INI1, viral protein synthesis and efficiency of processing.