Figure 4

Method of T cell stimulation determines HIV-1 susceptibility. (A) Comparison of viral replication in T_N, T_CM and T_EM cells that were stimulated by DC or by CD3/CD28 crosslinking with antibodies. The T cells were stimulated for 4 days, harvested and re-plated before infection with X4 HIV-1. Viral spread was followed by CA-p24 ELISA, of which the results of day 6 are shown. (B) To measure T cell proliferation T_N, T_CM or T_EM cells were incubated with DC or α-CD3/CD28 antibodies and after 4 days, cellular proliferation was determined by ³H-thymidine incorporation. Error bars represent standard deviations. * p < 0.05 ; ** p < 0.01; *** p < 0.001.