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Table 2 An example of the data used to estimate the proportion of cells that were resistant to puromycin.

From: A method to estimate the efficiency of gene expression from an integrated retroviral vector

Proportion of puromycin resistant cells seeded (P0)

Log(P0)

Proportion of peak count (C T )

Log(C T )

1/1024

-3.010

0.3896

-0.4093

1/4100

-3.612

0.5368

-0.2701

1/16400

-4.214

0.0871

-1.059

1/66000

-4.816

0.0559

-1.252

  1. The table shows the data obtained 21 days after cells were cultured in the presence of puromycin. Cultures containing different proportions of puromycin resistant cells and untransduced Jurkat cells (P0) were mixed and cultured under puromycin selection. The cells were counted daily, and the cell count at day 21 was expressed as a proportion of the peak count reached by the culture (C T ). The data was plotted onto a graph (Figure 4). The data in this table are not directly comparable to that shown in figure 3 because the total numbers of cells used in the calibration cultures were different.