From: A method to estimate the efficiency of gene expression from an integrated retroviral vector
Dilution | (1) | (2) | (3) | (4) | (5) | (6) | (7) |
---|---|---|---|---|---|---|---|
Dilution from (1) (d) | Â | 2 | 10 | 20 | 100 | 200 | 1000 |
Number of wells in which cell growth occurred | 30 | 30 | 18 | 18 | 19 | 13 | 3 |
Number of wells in which cell growth did not occur | 0 | 0 | 0 | 0 | 5 | 17 | 27 |
Proportion of wells that did not result in cell growth (f(x)) | 0/30 | 0/30 | 0/18 | 0/18 | 5/24 = 0.208 | 17/30 = 0.566 | 27/30 = 0.9 |
Mean number of cells (m = -ln f(x)) | Â | Â | Â | Â | 1.568 | 0.567 | 0.105 |
Estimated average number of cells per well in dilution (1) (m × d) |  |  |  |  | 156.8 | 113.4 | 105 |