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Figure 5 | Retrovirology

Figure 5

From: SUV39H1 interacts with HTLV-1 Tax and abrogates Tax transactivation of HTLV-1 LTR

Figure 5

SUV39H1 represses Tax transactivation of HTLV-1 LTR promoter activity. Representative results of luciferase assays using HEK293 and Jurkat cells (left and right panels, respectively) are shown with the mean and standard deviation of triplicate experiments. Below the graphs, results of immunoblot analyses of whole cell lysates are shown to confirm expression of transduced proteins. (a) Dose-dependent repression of Tax transactivation of HTLV-1 LTR by SUV39H1. More than three independent assays were done for each cell line. (b) Effects of SUV39H1 on the basal activities of HTLV-1 LTR. In the absence of Tax, increasing amounts of SUV39H1 expression plasmid was transfected with HTLV-1 Luc. Left and right panels show the results of HEK293 and Jurkat cells, respectively. (c) Absence of repression of Tax transactivation by HMTase negative SUV39H1. Tax expression plasmid was co-transfected with the wild type or HMTase negative mutant SUV39H1 along with the reporter plasmid pHTLV LTR-Luc. Lower two panels show the results of immunoblot analyses to confirm the expression of transduced Tax and SUV39H1 proteins. Antibodies used are indicated on the left. (d) Suppressive activities of SUV39H1 mutants on Tax transactivation of HTLV-1 LTR promoter activity. Fold activation of HTLV LTR promoter activity by Tax is shown with the mean and standard deviation of triplicated experiments. Co-transfected HA-tagged mutant SUV39H1 constructs are indicated below the graph and on the right of lower panels. Structures of these deletion mutants are described in Fig. 2a, upper panel.

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