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Figure 4 | Retrovirology

Figure 4

From: SUV39H1 interacts with HTLV-1 Tax and abrogates Tax transactivation of HTLV-1 LTR

Figure 4

Results of in vitro methyltransferase assays. (a) Time course analysis. Top panel shows a representative fluorogram of the reaction mixtures at the indicated time points analyzed by 15% SDS-PAGE. The middle panel shows the relative levels of methylation measured by densitometric analyses of the bands. Bottom panel, a result of immunoblot analysis of transduced SUV39H1 by anti-SUV39H1 monoclonal antibody, showing comparable levels of SUV39H1 expression in each sample. (b) A representative result of three independent experiments of in vitro methyltransferase assays of SUV39H1 transduced with or without Tax. The reaction time was 30 min. The second panel shows the relative intensities of the methylated H3 bands. Lower panels show the results of immunoblot analyses of the immunoprecipitates and whole cell lysates to show the presence of SUV39H1 with or without Tax. IP, immunoprecipitation; IB, immunoblot. Antibodies used are indicated on the side of the panels.

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