Figure 4
Both Rpt1 and Rpt2 domains of INI1 are required for the efficient activation of Tat-mediated HIV-1 transcription. a. Schematic representation of HA-tagged full-length INI1 and its mutants. b. 293T cells (2 × 104 cells per well) were cotransfected with 100 ng of pHIV-1-LTR-Luc [25], 50 ng of pcDNA3-Tat101-FLAG [41], and/or 200 ng of pCGN-INI1 or its mutant (20.2, 1.2, 3B, 27B) [7] in triplicate and luciferase assays were performed 24 h later. c. 293T cells were cotransfected with 5 μg of pcDNA3-Tat101-FLAG and/or 5 μg of pCGN-HA-INI1 or its mutant (20.2, 3B, 27B) and IP-western blotting was performed in the similar way as shown in Fig. 3. Asterisk denotes HA-INI1 or its mutant.