Skip to main content
Figure 4 | Retrovirology

Figure 4

From: Candidate polyanion microbicides inhibit HIV-1 infection and dissemination pathways in human cervical explants

Figure 4

Inhibition of HIV-1 binding and transfer through DC-SIGN by polyanions. DC-SIGN+ Raji cells were treated with test compound (A) PRO 2000 or B) Dextrin Sulphate for 1 hour prior to exposure to HIV-1 (i) RF or (ii) BaL for 2 hours in the presence of compound. Following removal of excess compound and virus, cells were either lysed for analysis by p24 ELISA to determine inhibition of viral binding (■), or co-cultured with permissive T cells (PM-1) to determine inhibition of viral transfer (). Background binding to Raji cells was determined using Raji/DC-SIGN- cells, or 100 μg/ml mannan. Data represent the mean ± SEM of 3 independent experiments (except PRO 2000 against RF, where n = 2) where each condition was tested in triplicate. Statistical analysis was completed using student's T-test with statistically significant changes marked * (p < 0.05), ** (p < 0.01) or *** (p < 0.005). ND = not determined.

Back to article page