Figure 3
Polyanion inhibition of HIV-1 BaL infection of cervical explants and transfer of virus from migratory cells. Ectocervical explants were exposed to HIV-1BaL for 2 hours in the presence of test compound. Following overnight culture, explants were separated from any cells that had migrated from the tissue and cultured separately. (i) Infection of cervical explants was determined by ELISA measurement of p24 antigen in culture supernatants. (ii) Migratory cells were co-cultured with permissive T cells (PM-1) and infection determined by p24 antigen in culture supernatants. Data represent the % HIV-1 infection observed following compound treatment when compared to tissue exposed to virus alone. Each compound was tested using n = 3 – 8 independent donors, where each condition was tested in triplicate. Compounds tested were A) PRO 2000 and B) Dextrin Sulphate. Inserted figures represent the mean ± SEM concentration inhibiting 50% HIV-1 infection (IC50) for each compound. Statistical analysis was completed using student's T-test with statistically significant changes marked * (p < 0.05), or *** (p < 0.005).