Figure 1

Inhibitory effect of polyanionic compounds against HIV-1 infection of T-cells. HIV-1 BaL (R5, ■, solid line) or RF (X4, □, dotted line) was immobilised onto solid phase using anti-HLA-DR antibody capture, as described in the Methods. (i) Direct virucidal activity was determined by the pre-treatment of immobilised virus for 1 hour before culture with target PM-1 cells in the absence of compound. (ii) Receptor mediated blockade activity was determined by the pre-treatment of target PM-1 cells (1 hour) prior to exposure to immobilised virus in the absence of compound. (iii) Attachment/fusion inhibition was determined by the pre-treatment of immobilised virus with test compound prior to the addition of target PM-1 cells in the presence of compound. Plates were cultured for 10 days following which viral replication was determined by reverse transcriptase measurement of culture supernatants. Compounds tested were: A) PRO 2000; and B) Dextrin sulphate. Data represent the mean ± SEM of n = 5 (PRO 2000) or 4 (Dextrin sulphate) independent experiments where each condition was tested in triplicate. Inserted figures represent the mean ± SEM concentration inhibiting 50% infection (IC₅₀) for compounds against each virus.