Recruitment of CREB and basal transcription machinery at the Sgt1 and p97/Vcp promoters. A) Presence of Pol II on Sgt1 promoter. ChIP analysis of Sgt1 promoter using IgG, anti- H3S10, and anti- Pol II antibodies (α-RNAP II, Santa Cruz, polyclonal rabbit #N-20)compared to input (lanes 1, 2, 3 and 5, respectively). IgG and beads only (lanes 1 and 4) were used as negative controls. Top panel corresponds to typical results from CTLL/WT cells, while the bottom panel corresponds to results from CTLL/703 cells. B) Presence of CREB and Pol II on Sgt1 and p97 promoters. ChIP analysis of Sgt1 and p97/Vcp promoters using antibodies against CREB, p-CREB and Pol II (lanes 2, 3 and 4). The Pol II antibody recognizes the phosphorylated elongating Pol II complex (α-Ser 2P CTD (H5, Covance)). IgG and Beads alone (lanes 1 and 5) were used as negative controls.