Figure 2

Overview of microarray analysis, annotation, and promoter analysis. A schematic depicting the workflow of the project. Gene expression analysis of CTLL/WT and CTLL/703 cells was performed utilizing the Affymetrix's Murine Genome U74A GeneChip. Genes that were either up-or down-regulated in CTLL/WT cells by a magnitude of at least two-fold were functionally annotated using NIH's DAVID bioinformatics program. Next, promoter sequences (2100 bp surrounding the predicted TSS) were retrieved from PromoSer. One third of the promoter sequences retrieved were checked for proper alignment against the mouse genome using Blastn and MapViewer tools through NCBI. CREB (TGACGT/C, A/GGGAGT) consensus sequences were obtained through TRANSFAC database and searched within the promoters obtained. Factors that contained the CREB sequences within their promoters were further probed for genes that contribute directly to mitosis, cytokinesis, and microtubule organization.