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Figure 5 | Retrovirology

Figure 5

From: Intracytoplasmic maturation of the human immunodeficiency virus type 1 reverse transcription complexes determines their capacity to integrate into chromatin

Figure 5

Quantitative PCR analysis of ERT activity and integration of cytoplasmic and nuclear RTCs. A. ERT activity of cRTCs and nRTCs isolated 2 h and 5 h post-infection. cRTCs and nRTCs were normalized according to strong-stop (early) HIV-1 DNA content measured by real-time PCR. ERT reaction was performed in duplicate as described in the text. HIV-1 DNA was quantified by real-time PCR. HIV-1 DNA in RTCs incubated without dNTPs (control) was taken as 100%. Results are presented as mean ± SE. B. Quantitative PCR analysis of PIC integration into chromatin. cPICs and nPICs after the ERT reaction performed with or without (control) dNTPs were incubated in triplicate with chromatin samples. DNA was purified and analyzed by Alu-LTR-based real-time nested PCR [29]. Integration efficiency was evaluated relative to integration of cPIC isolated 2 h p.i. Results are presented as mean ± SD.

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Retrovirology

ISSN: 1742-4690

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