Figure 3From: Intracytoplasmic maturation of the human immunodeficiency virus type 1 reverse transcription complexes determines their capacity to integrate into chromatinNuclear translocation of RNA and DNA containing HIV-1 PICs. DNA and RNA were purified from cytoplasmic and nuclear HIV-1 complexes 5 h after infection of HeLa cells in the presence or absence of AZT (3 μM). Triplicate samples were analyzed by real-time PCR with primers specific for late HIV-1 DNA by measuring SYBR Green fluorescence. Results are presented as mean ± SD. A. Absolute values of nuclear and cytoplasmic HIV-1 DNA and RNA in RTCs. B. Percentage of nuclear RNA or DNA relative to cytoplasmic RNA or DNA, respectively.Back to article page