Figure 7

Inhibition of trans-infection by MDDCs and of Fc-gp120 binding to primary PBLs and MDDCs. A) MAbs b12 or 2G12, or mannan were incubated with virus (10 and 50 ng p24). The mixture was then incubated with primary MDCCs, followed by washing. Cells were then added on top of TZM-BL cells previously plated a day before, and trans-infection was assayed two days later by a β-gal assay. Results are the mean values of triplicates. B) Inhibition of Fc-gp120 binding to primary PBLs and MDDCs in the presence of sCD4, b12 Fab, 2G12 Fab or mannan at the concentrations indicated. Inhibition is expressed as the percentage of Fc-gp120 binding.