Figure 5

Effect of 2G12 on Env-HSPG and Env-DC-SIGN binding. A) The inhibitory effect of mannan (50 μg/ml) and 2G12 Fab (25 μg/ml) and 2G12 IgG (25 μg/ml) on binding of Fc-gp120 and gp120-Fc chimeras to CHO pgsA745 cells transduced with MIGR1 GFP/DC-SIGN. Fc alone was included as a negative control. Data are representative of triplicates. B) Namalwa cells expressing either CD4, HSPGs (syndecan 2) or DC-SIGN were assessed for Fc-gp120 binding in the absence or presence of varying concentrations of mAbs b12 and 2G12. Inhibition is expressed as a percentage of Fc-gp120 binding compared to controls. C) Virus capture by Namalwa cells expressing either HSPGs or DC-SIGN was assessed in the absence or presence of varying concentrations of mAbs b12 and 2G12. Inhibition is expressed as percentage of p24 captured compared to controls. Results are representative of two experiments.