Skip to main content

Table 2 Kinetic parameters for HIV-1 sIN substituted proteins binding to DNA substrates

From: Mode of inhibition of HIV-1 Integrase by a C-terminal domain-specific monoclonal antibody*

 

U5-End

U5-End Cut

Target

 

kon 105 M-1 s-1

koff 10-3 s-1

Kd (nM)

kon 105 M-1 s-1

koff 10-3 s-1

Kd (nM)

kon 105 M-1 s-1

koff 10-3 s-1

K d (nM)

sIN

1.7 ± 0.1

1.0 ± 0.1

5.9 ± 0.7

1.1 ± 0.1

1.3 ± 0.1

11.8 ± 1.4

1.0 ± 0.1

1.6 ± 0.2

16.0 ± 2.6

F223A

2.1 ± 0.2

1.1 ± 0.1

5.2 ± 0.7

1.1 ± 0.2

1.6 ± 0.2

14.5 ± 3.2

0.8 ± 0.1

2.5 ± 0.2

31.3 ± 4.7

R224A

1.3 ± 0.2

0.9 ± 0.1

6.9 ± 1.3

1.1 ± 0.2

1.2 ± 0.2

10.9 ± 2.7

0.9 ± 0.2

1.5 ± 0.2

16.7 ± 2.3

Y226A

1.2 ± 0.2

1.0 ± 0.1

8.3 ± 1.6

0.8 ± 0.1

0.6 ± 0.1

7.5 ± 1.6

0.6 ± 0.1

0.7 ± 0.1

11.7 ± 2.6

I267A

0.9 ± 0.1

0.2 ± 0.1

2.2 ± 1.1

1.0 ± 0.1

0.3 ± 0.1

3.0 ± 1.0

0.8 ± 0.1

0.3 ± 0.1

3.8 ± 1.6

I267A/I268A

0.8 ± 0.1

0.2 ± 0.1

2.5 ± 1.3

0.9 ± 0.1

0.3 ± 0.1

3.3 ± 1.2

0.7 ± 0.1

0.2 ± 0.1

2.9 ± 1.5

K244A

1.9 ± 0.2

1.2 ± 0.2

6.3 ± 1.2

0.9 ± 0.1

1.0 ± 0.2

11.1 ± 2.5

0.9 ± 0.1

1.2 ± 0.2

13.3 ± 2.7

W243A

0.5 ± 0.1

0.7 ± 0.1

14.0 ± 3.4

0.8 ± 0.2

0.9 ± 0.1

11.3 ± 0.3

0.9 ± 0.1

1.4 ± 0.1

15.6 ± 2.1

R262A

-

-

ND

-

-

ND

-

-

ND

  1. Three different model biotin-labeled substrates were immobilized on the surface of a SA chip as described in Experimental Procedures. BIAcore data were collected by injecting different concentrations of sIN, ranging from 50 to 300 nM dimers, at a flow rate of 30 μL/min. All data were collected using buffer containing 5 mM MgCl2. The dissociation constant was calculated from the ratio of the average of the off and the apparent on rates. All injections were done at 25°C. ND, kinetic parameters could not be determined due to low binding efficiency.
Back to article page

Retrovirology

ISSN: 1742-4690

Contact us