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Figure 5 | Retrovirology

Figure 5

From: Interactions between Nef and AIP1 proliferate multivesicular bodies and facilitate egress of HIV-1

Figure 5

Interactions between Nef and AIP1 are required for the proliferation of MVBs. HeLa.CIITA cells were transfected with plasmids, which directed the expression of GFP, Nef.GFP, or mutant NefYPL.GFP chimeras (top panels). GFP-positive cells were isolated by FACS and fixed before ultra-thin sectioning was performed. MVBs were identified by their unique morphology (bottom left panel) under the electron microscope (indicated by arrows). Numbers of MVBs of each cell type were counted directly under the electron microscope from 30 profiles randomly taken from each sample. Bar graphs contain: White bars, GFP control; black bars, Nef; striped bars, mutant Nef.YPL protein.The black bar inside the EM panels measures 1 μm.

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