Figure 3
From: Interactions between Nef and AIP1 proliferate multivesicular bodies and facilitate egress of HIV-1
Nef contains the consensus-binding site for AIP1. Multiple alignments of sequences were generated by the Clustal W software and visually inspected for the presence of already described L domain motifs [47]. The AIP1-consensus binding site is highlighted. Consensus residues represent several subtypes of HIV-1. Below them are Nef sequences from HIV-2 and SIV that do not contain this consensus sequence. AIP1 binds elsewhere on these proteins. These sequences are from the Los Alamos database [48]. Below these sequences are diagrammed mutations that were introduced into Nef, one mutating the YPL sequence to three alanines (NefYPL), the other deleting the entire consensus motif (NefΔ YPL).