Cyclin T1 expression is induced in MM6 cells through a post-transcriptional mechanism. (A) Cell extracts were prepared from untreated MM6 cells or MM6 cells treated with PMA from 1 to 48 hours as indicated. Immunoblots were performed to measure levels of Cyclin T1 (CycT1), CDK9 and β-actin proteins. (B) Cell extracts were prepared from untreated MM6 cells (Con) or MM6 cells treated with PMA, vitamin D3 (VitD), retinoic acid (RA), LPS, or interferon gamma (IFNγ) for 24 or 48 hours. Immunoblots were performed to measure levels of Cyclin T1, CDK9 and β-actin proteins. (C) Total RNA was isolated from untreated MM6 cells or cells treated with PMA for 24 hours. Quantitative real-time RT-PCR was used to measure the expression level of Cyclin T1, CDK9, and CD11c mRNA. The fold-change represents the change of transcript levels in PMA-treated MM6 cells relative to untreated cells after normalization to β-actin mRNA levels which are insensitive to PMA.