| Â | Mutation | LTR | NFkB |
|---|
2B | None | 100% | 100% |
| Â | G21D | 26% | 138% |
| Â | L87I | < 10 % | 90% |
| Â | P92L | 24% | 86% |
| Â | Y144C | 58% | 65% |
| Â | Y144R | < 5% | < 5% |
| Â | A204T | 41% | 54% |
| Â | W248R | < 5% | < 5% |
| Â | L308V | 66% | 88% |
Mo | None | 41% | 73% |
- Jurkat cells were co-transfected with either wildtype Tax 2B or Tax 2B mutants together with 1 ug of the LTR or NFkB luciferase reporters and 50 ng of the control Renilla plasmid pRL TK. After 24 hrs the cells were lysed and reporter activities were measured using a Turner 20/20 Luminometer and firefly reporter activities were normalised using Renilla luciferase values. Normalised values for wildtype Mo and mutant Tax proteins were calculated as a percentage of wildtype 2B values which was set at 100%.
