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Table 3 Transactivation phenotypes of Tax 2A Mo and Tax 1 mutants

From: Functional analysis of human T lymphotropic virus type 2 Tax proteins

 

Mutation

LTR

NFkB

Mo

None

100%

100%

 

G21D

14%

140%

 

L87I

< 5%

125%

 

P92L

13%

120%

 

Y144C

< 5%

< 5%

 

Y144R

< 5%

< 5%

 

T204A

300%

168%

 

W248R

10%

< 5%

 

L308V

75%

80%

2B

None

246%

195%

Tax 1

None

100%

100%

 

Y144C

55%

36%

 

W248R

< 5%

< 5%

  1. Jurkat cells were co-transfected with the indicated Tax plasmids together with 1 ug of the LTR or NFkB luciferase reporters and 50 ng of the control Renilla plasmid pRL TK. After 24 hrs the cells were lysed and reporter activities were measured using a Turner 20/20 Luminometer and firefly reporter activities were normalised using Renilla luciferase values. Normalised values for wildtype Tax 2B and mutant Tax proteins were calculated as a percentage of the corresponding wildtype Mo or Tax 1 values which was set at 100%.