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Table 2 Transactivation phenotypes of Tax 2A Lor mutants

From: Functional analysis of human T lymphotropic virus type 2 Tax proteins

 

Mutations

LTR

NFkB

Mo

None

100%

100%

Lor

G21D/L87I/P92L/T204A/W248R/L308V

< 5%

< 5%

L1

L87I/P92L/T204A/W248R/L308V

< 5%

< 5%

L2

P92L/T204A/W248R/L308V

< 5%

< 5%

L3

T204A/W248R/L308V

< 5%

< 5%

L4

W248R/L308V

< 5%

< 5%

L5

L308V

75%

80%

L6

G21D/L87I/P92L/T204A/L308V

< 5%

150%

  1. Jurkat cells were co-transfected with either 250 ng of Mo wildtype, Lor or Lor mutants (L1–L6) together with 1 ug of the LTR or NFkB luciferase reporters and 50 ng of the control Renilla plasmid pRL TK. After 24 hrs the cells were lysed and reporter activities were measured using a Turner 20/20 Luminometer. Firefly reporter activities were normalised using Renilla luciferase values. Normalised values for mutant Tax proteins were calculated as a percentage of wildtype Mo values which was set at 100%.
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Retrovirology

ISSN: 1742-4690

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